TDP-43, Abcam, ab133547

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human TCP-43 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 18 commercial research antibodies against human TDP-43 encoded by TARDBP

The collection of antibodies was tested using HAP1 WT and TARDBP KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ab133547 detected a band of appr. 45 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. No band was detected following IP (2 µg Ab used). In IF (1:700 ab dilution) clear staining was observed in WT cells and KO cells showed no stain.

We highly encourage other scientists to share their observations.