TDP-43, Thermo Fisher Scientific, MA5-27828

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human TCP-43 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 18 commercial research antibodies against human TDP-43 encoded by TARDBP

The collection of antibodies was tested using HAP1 WT and TARDBP KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) MA5-27828 detected a band of appr. 45 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was detected following IP (2 µg Ab used), this band was more intense compared to band in input extract (4% of starting material). A faint band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from cell lysate. In IF (1:1000 ab dilution) clear staining was observed in WT cells and KO cells showed no stain.

We highly encourage other scientists to share their observations.