TAF15, Bio-Techne, NBP1-92685

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human TATA-box binding protein-associated factor 15 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 5 commercial research antibodies against human TATA-box binding protein-associated factor 15 encoded by TAF15.

The collection of antibodies was tested using HAP1 WT and TAF15 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) NBP1-92685 detected a band of appr. 75 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Furthermore, an intense band of lower molecular weight was observed in both genotypes. A band was detected following IP (2 μg ab used), however the intensity was comparable to band in input extract (4% starting material) and unbound fraction (4% of unbound fraction), showing that some antigen is precipitated but not completely. In IF (1:1000 Ab dilution), a more intense staining was observed in WT cells compared to KO cells showing no background.

We highly encourage other scientists to share their observations.