Synaptotagmin-1, Bio-Techne, MAB4364

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Synaptotagmin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Synaptotagmin-1 encoded by SYT1.

The collection of antibodies was tested using HCT116 WT and SYT1 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate), IF (WT and KO cells) and flow cytometry (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) MAB4364 detected a band of appr. 63 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A strong band was detected following immunoprecipitation (2 μg ab used), with substantial enrichment relative to the starting material (4%). The signal was highly concentrated in the IP fraction, indicating efficient capture of the target protein by the antibody. Only a faint residual signal was observed in the unbound (4%) fraction, suggesting that most of the antigen was removed from the lysate during immunoprecipitation. In IF (1:500 ab dilution), staining intensity varied among WT cells, with some cells exhibiting markedly stronger staining than KO cells showing only background signal. Flow cytometry using MAB4364 (1 μg/mL antibody) showed a right-shifted fluorescence intensity distribution in WT cells compared with KO cells. The KO population largely overlapped with the secondary-only control, consistent with low background staining.