SYK, Developmental Studies Hybridoma Bank, AFFN-SYK-5A10

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Tyrosine-protein kinase SYK and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Tyrosine-protein kinase SYK encoded by SYK.

The collection of antibodies was tested using THP-1 WT and SYK KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 ab dilution) AFFN-SYK-5A10 did not detect a band. AFFN-SYK-5A10 (2 μg ab used) was not able to immunocapture tyrosine-protein kinase SYK. In IF (1:60 ab dilution), comparable staining intensity was observed in KO and WT cells.