Syntaxin-4, Thermo Fisher Scientific, PA5-51560

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Syntaxin-4 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 9 commercial research antibodies against human Syntaxin-4 encoded by STX4.

The collection of antibodies was tested using HAP1 WT and STX4 KO cells in the applications WB (WT and KO cell lysates/60 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) PA5-51560 detected a band of appr. 34 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band of higher molecular weight was observed in both genotypes. A band was detected following IP (2 µg Ab used), this band was markedly more intense compared to band in input extract (10% of starting material) and no band was detected in immunodepleted extract (10% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. In IF (1:300 ab dilution), a more intense staining intensity was observed in WT cells compared to KO cells showing some background.