Sequestosome-1, GeneTex, GTX629890

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Sequestosome-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 17 commercial research antibodies against human Sequestosome-1 encoded by SQSTM1.

The collection of antibodies was tested using U2OS WT and SQSTM1 KO cells in the applications WB (WT and KO cell lysates/25 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) GTX629890 detected a band of appr. 60 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. No band was detected following IP (1 µg ab used). In IF (antibody dilution 1:1000), clear staining was observed in WT cells, whereas KO cells showed no staining.