SOD1, Thermo Fisher Scientific, MA1-105

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Superoxide dismutase [Cu-Zn] 1 (SOD1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 11 commercial research antibodies against human Superoxide dismutase [Cu-Zn] 1 (SOD1) encoded by SOD1.

The collection of antibodies was tested using HeLa WT and SOD1 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) MA1-105 detected a band of appr. 16 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. No band was detected following IP (2 µg Ab used). In IF (1:1000 ab dilution), comparable staining intensity was observed in both KO and WT cells.