acid sphingomyelinase, Proteintech, 14609-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human acid sphingomyelinase (ASM) protein and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 9 commercial research antibodies against human acid sphingomyelinase (ASM) protein encoded by SMPD1.

The collection of antibodies was tested using U-87 MG WT and SMPD1 KD cells in the applications WB (WT and KO culture medium/10 µg protein and WT and KO cell lysate/30 µg protein) and IP (WT culture medium) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) 14609-1-AP detected no band specific to WT culture medium. This pattern was consistent in cell lysate. Following IP (2 µg antibody used), a band was detected at the expected molecular weight. The band was only slightly more intense in the IP fraction than in the input extract (4% of starting material) and the immunodepleted extract (4% of the total unbound fraction), indicating that the antibody did not efficiently deplete the antigen from the cell lysate.