SGLT2, Santa Cruz Biotechnology, sc-393350

We share a literature review of commercially available SGLT2 antibodies evaluated by immunohistochemistry and western blotting using kidneys and hearts from WT and SGLT2 deficient mice and rats. Antibody specificity was also evaluated in human kidney (Hirose et al., Hypertension, 2026). The authors perform a head-to-head screen of a panel of eight commercial SGLT2 antibodies.
We have rated the antibody performance according to the pAbmAbs criteria, and we highly encourage other scientists to share their experiences.
The antibody sc-393350 showed showed unspecific immunostaining in kidney sections from WT mice and rats (Figure 1) with similar staining observed WT compared to Sglt2-/- sections from mouse and rat. Furthermore, western blotting with this antibody resulted in several unspecific bands using mouse and rat kidney homogenates as samples. These bands were also present in Sglt2-/- samples (Figure 4).