SGLT2, BiCell Scientific, 20802

We share a literature review of commercially available SGLT2 antibodies evaluated by immunohistochemistry and western blotting using kidneys and hearts from WT and SGLT2 deficient mice and rats. Antibody specificity was also evaluated in human kidney (Hirose et al., Hypertension, 2026). The authors perform a head-to-head screen of a panel of eight commercial SGLT2 antibodies.
We have rated the antibody performance according to the pAbmAbs criteria, and we highly encourage other scientists to share their experiences.
The antibody 20802 showed specific immunostaining in kidney sections from WT mice and rats (Figure 1) not observed in Sglt2-/- sections from mouse and rat. However, a high background signal was observed in both species.
Western blotting with this antibody detected a distinct band of the expected size using mouse and rat kidney homogenates as samples. However, a weak band of higher molecular weight was observed in mouse but not rat WT and Sglt2-/- samples (Figure 4).