SGLT2, Abcam, ab306558

We share a literature review of commercially available SGLT2 antibodies evaluated by immunohistochemistry and western blotting using kidneys and hearts from WT and SGLT2 deficient mice and rats. Antibody specificity was also evaluated in human kidney (Hirose et al., Hypertension, 2026). The authors perform a head-to-head screen of a panel of eight commercial SGLT2 antibodies.
We have rated the antibody performance according to the pAbmAbs criteria, and we highly encourage other scientists to share their experiences.
The antibody ab306558 showed specific immunostaining in kidney sections from WT mice and rats (Figure 1) and no staining in cardiac sections. No staining was observed in Sglt2-/- sections from mouse and rat, confirming the specificity. However, no specific signal could be detected in human kidneys.
Western blotting with this antibody detected a distinct band of the expected size using mouse and rat kidney homogenates as samples. No unspecific bands were observed in Sglt2-/- samples (Figure 4).