Syndecan-4, Bio-Techne, AF2918

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Syndecan-4 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Syndecan-4 encoded by SDC4.

The collection of antibodies was tested using MCF7 WT and SDC4 KD cells in the applications WB (WT and KO cell lysates/50 µg protein) and IP (WT culture media) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) AF2918 detected two bands of appr. 32 and 36 kDa in WT cell lysate with no corresponding bands observed in KD cell lysate. A band was detected following IP (2 ug Ab used), and no band was detected in starting material or unbound fraction, showing that the antibody does deplete the antigen from culture media.

Human Syndecan-4 Antibody (R&D Systems, AF2918)

Detects faint bands in HepG2 cell lysate. The intensities are not affected by heparinase treatment of the cells prior to lysis. No bands were identified in liver homogenate from mice. I have used the antibody concentration recommended by the supplier, but the results might be improved with higher concentration.
Loaded 40 ug protein
Used 0.1 ug/ml