Steroyl-CoA desaturase, Thermo Fisher Scientific, MA5-27542

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Stearoyl-CoA desaturase (SCD1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 9 commercial research antibodies against human SCD1 endoced by SCD.

The collection of antibodies was tested using HeLa WT and SCD KO cells in the applications WB (WT and KO cell lysates/35 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) MA5-27542 detected a band of appr. 35 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was detected following IP (2 µg Ab used), this band had same intensity compared to bands in input extract (4% of starting material) and immunodepleted extract (4% of total unbound fraction), showing that the antibody does not deplete the antigen from cell lysate. In IF (1:1000 ab dilution), a clear intense staining was observed in WT cells with no observed background in KO cells.