Steroyl-CoA desaturase, Bio-Techne, AF7550

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Stearoyl-CoA desaturase (SCD1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 9 commercial research antibodies against human SCD1 endoced by SCD.

The collection of antibodies was tested using HeLa WT and SCD KO cells in the applications WB (WT and KO cell lysates/35 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 ab dilution) AF7550 detected a band of appr. 35 kDa in WT cell lysate not observed in KO cell lysate. A band was detected following IP (2 µg Ab used), this band was more intense compared to bands in input extract (4% of starting material) and immunodepleted extract (4% of total unbound fraction). In IF (1:200 ab dilution), a clear intense staining was observed in WT cells with no observed background in KO cells.