sphingosine 1-phosphate receptor 1, ABclonal, A12935

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Sphingosine 1-phosphate receptor 1 (S1PR1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 9 commercial research antibodies against human S1PR1.

The collection of antibodies was tested using SK-HEP-1 WT and S1PR1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) A12935 detected a band of appr. 48 kDa in WT cell lysate with a faint corresponding band observed in KO cell lysate. Furthermore, an intense band of higher molecular weight was observed in both genotypes along with bands of lower molecular weight.
A smear was detected following IP (2 µg Ab used), this smear was more intense compared to band in input extract (4% of starting material). A faint smear was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from cell lysate. In IF (1:1000 ab dilution), comparable staining intensity was observed in both KO and WT cells.