Rab13, Thermo Fisher Scientific, PA5-52039

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab13 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 11 commercial research antibodies against human Rab13

The antibody panel was evaluated across multiple applications. For Western blotting (WB), HEK-293T WT and RAB13 KO lysates (30 µg protein) as well as U-87 MG WT and RAB13 KD lysates (40 µg protein) were analyzed. U-87 MG lysates were used for immunoprecipitation (IP), while U-87 MG control and RAB13 KD cells were used for immunofluorescence (IF).

In WB (1:1000 ab dilution) on both HEK-293T and U-87 MG PA5-52039 detected a band of appr. 24 kDa with no corresponding band observed in either KD or KO cell lysate. A band was detected following IP (2 µg Ab used), this band was more intensity compared to bands in input extract (4% of starting material). A band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not deplete the antigen from cell lysate. In IF (1:1000 ab dilution), a more intense staining was observed in WT cells compared to KD cells showing some background.

We highly encourage other scientists to share their observations.