Rab13, R&D Systems, MAB8305

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab13 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 11 commercial research antibodies against human Rab13

The antibody panel was evaluated across multiple applications. For Western blotting (WB), HEK-293T WT and RAB13 KO lysates (30 µg protein) as well as U-87 MG WT and RAB13 KD lysates (40 µg protein) were analyzed. U-87 MG lysates were used for immunoprecipitation (IP), while U-87 MG control and RAB13 KD cells were used for immunofluorescence (IF).

In Western blotting (WB; 1:200 antibody dilution) using HEK-293T cells, MAB8305 detected a band at approximately 24 kDa in WT lysates, with no corresponding band observed in RAB13 KO lysates. Bands at higher molecular weight were detected in both genotypes. In U-87 MG cells, MAB8305 detected a band at approximately 24 kDa in WT lysates, with no corresponding band in RAB13 KD lysates. Bands of higher molecular weight was observed in both genotypes. Only a faint band was detected following IP (2 µg Ab used). In IF (1:500 ab dilution) a more intense staining was observed in WT cells compared to KD cells showing some background

We highly encourage other scientists to share their observations.