Rab13, Abcam, ab233810

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab13 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 11 commercial research antibodies against human Rab13

The antibody panel was evaluated across multiple applications. For Western blotting (WB), HEK-293T WT and RAB13 KO lysates (30 µg protein) as well as U-87 MG WT and RAB13 KD lysates (40 µg protein) were analyzed. U-87 MG lysates were used for immunoprecipitation (IP), while U-87 MG control and RAB13 KD cells were used for immunofluorescence (IF).

In Western blotting (WB; 1:1000 antibody dilution) using HEK-293T cells, ab233810 detected a faint band at approximately 24 kDa in WT lysates, with no corresponding band observed in RAB13 KO lysates. Intense bands at higher molecular weights were detected in both genotypes. In U-87 MG cells, ab233810 detected a band at approximately 24 kDa in WT lysates, with no corresponding band in RAB13 KD lysates. Intense smearing at higher molecular weights was observed in both genotypes. Only a faint band was detected following IP (2 µg Ab used). In IF (1:1000 ab dilution) a more intense staining was observed in WT cells with some background staining observed in KD cells.

We highly encourage other scientists to share their observations.