Rab10, Abcam, ab181367

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab10 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human Rab10 encoded by RAB10

The collection of antibodies was tested using HAP1 WT and RAB10 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ab181367 detected a band of appr. 23 kDa was observed in WT cell lysate with no corresponding band observed in KO cell lysate. An intense band of higher molecular weight was observed in both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:100 ab dilution), comparable staining intensities was observed in both KO and WT cells.

We highly encourage other scientists to share their observations.