Rab8A, Thermo Fisher Scientific, PA5-72605

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab8A and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Rab8A encoded by RAB8A.

The collection of antibodies was tested using HeLa WT and RAB8A KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) PA5-72605 detected a band of appr. 24 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Multiple bands (<5) of strong intensity were observed in both genotypes. A band was detected following IP (2 µg Ab used), this band was markedly more intense compared to band in input extract (4% of starting material). A faint band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from cell lysate. In IF (1:250 ab dilution), a more intense staining was observed in WT cells compared to KO cells showing some background.

We highly encourage other scientists to share their observations.