Rab8A, Thermo Fisher Scientific, MA5-31883

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab8A and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Rab8A encoded by RAB8A.

The collection of antibodies was tested using HeLa WT and RAB8A KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) MA5-31883 detected no band of interest. No band of interest was detected following IP (2 µg Ab used). In IF (1:500 ab dilution) a more intense staining was observed of KO cells compared to WT cells.

We highly encourage other scientists to share their observations.