Rab8A, Thermo Fisher Scientific, MA5-24717

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab8A and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Rab8A encoded by RAB8A.

The collection of antibodies was tested using HeLa WT and RAB8A KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) MA5-24717 detected a smear of bands, one of which were specific to WT cell lysate, with no corresponding band observed in KO cell lysate. No band of interest was detected following IP (2 µg Ab used). In IF (1:200 ab dilution) a more intense staining was observed in WT cells compared to KO cells showing some background.

We highly encourage other scientists to share their observations.