Rab7A, Thermo Fisher Scientific, PA5-52369

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab7A and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Rab7A encoded by RAB7A.

The collection of antibodies was tested using HeLa WT and RAB7A KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) PA5-52369 detected a band of appr. 24 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Bands of higher molecular weight was observed in both genotypes. A band was detected following IP (2 µg Ab used). This band had same intensity as band in input extract (4% of starting material) and immunodepleted extract (4% of total unbound fraction), showing that the antibody does not deplete the antigen from the cell lysate. In IF (1:200 ab dilution), comparable staining intensities were observed in both WT and KO cells.

We highly encourage other scientists to share their observations.