Rab5C, Proteintech, 27219-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab5C and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 3 commercial research antibodies against human Rab5C encoded by RAB5C.

The collection of antibodies was tested using HAP1 WT and RAB5C KO cells in the applications WB (WT and KO cell lysates/35 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) 27219-1-AP detected an intense band of appr. 24 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Furthermore, several bands of higher molecular weight were observed in both genotypes. No band was detected following IP (2 ug ab used). In IF (1:500 ab dilution) a more intense staining of WT cells was observed compared to KO cells showing some background.

We highly encourage other scientists to share their observations.