Rab3A, Thermo Fisher Scientific, MA5-37684

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab3A and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human Rab3A.

The collection of antibodies was tested using SK-N-FI WT and RAB3A KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) MA5-37684 detected a very faint band of appr. 25 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Faint bands of both higher and lower molecular weight were observed in both genotypes. No band was detected following IP (2 ug ab used). In IF (1:25 ab dilution), comparable staining intensity was observed in both KO and WT cells.

We highly encourage other scientists to share their observations.