Rab3A, Abcam, ab3335

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab3A and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human Rab3A.

The collection of antibodies was tested using SK-N-FI WT and RAB3A KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ab3335 detected a band of appr. 25 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Strong bands of higher molecular weight were observed in both genotypes. A band was detected following IP (2 ug ab used).This band was markedly more intense than the corresponding band in input extract (4% of starting material). No band was detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does completely deplete the antigen from the cell lysate. In IF (1:1000 ab dilution), a more intense staining was observed in WT cells compared to KO cells showing background staining.

We highly encourage other scientists to share their observations.