Rab1B, Thermo Fisher Scientific, PA5-77240

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab1A and Rab1B and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Rab1A and Rab1B.

The collection of antibodies was tested using HAP1 WT, RAB1A KO and RAB1B KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In Western blot (1:200 antibody dilution), PA5-77240 detects multiple bands > 31 kDa in all tested lysates. A weak band near the expected size (~24 kDa) is observed in WT lysate. The band appears stronger in Rab1A KO lysate and weaker in Rab1B KO cell, suggesting some specificity for Rab1B, however, the overall background signal is high, with substantial non-specific binding observed across all lanes. The antibody (2 µg) does not immunoprecipitate Rab1B from HAP1 WT cell lysates. Rab1B in the starting material, unbound fraction, and precipitate was detected by WB using 17824-1-AP. IF was performed on WT and Rab1B KO cells. An intense signal was observed in WT cells at a 1:50 Ab dilution, with some background signal in KO cells.

We highly encourage other scientists to share their observations.