Rab1B, Proteintech, 17824-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab1A and Rab1B and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Rab1A and Rab1B.

The collection of antibodies was tested using HAP1 WT, RAB1A KO and RAB1B KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In Western blot (1:500 antibody dilution), 17824-1-AP detects a strong band at the expected size (~22 kDa) in WT and Rab1A KO cell lysates, with only a faint corresponding signal in Rab1B KO cell lysates supporting Rab1B specificity. A weaker band above 57 kDa is observed in all tested lysates. The antibody (2 µg) does not immunoprecipitate Rab1B from HAP1 WT cell lysates. Rab1B in the starting material, unbound fraction, and precipitate was detected by WB using 17824-1-AP. IF was performed on WT and Rab1B KO cells. An intense signal was observed in WT cells at a 1:50 Ab dilution, with some background signal in KO cells.

We highly encourage other scientists to share their observations.