Rab1A, Thermo Fisher Scientific, PA5-104066

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab1A and Rab1B and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Rab1A and Rab1B.

The collection of antibodies was tested using HAP1 WT, RAB1A KO and RAB1B KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution), PA5-104066 detects a smeared signal with multiple bands. The most intense band appears just above the 31 kDa marker and is observed in all test lysates including lysate from Rab1A KO cells. The antibody (2 µg) does not immunoprecipitate Rab1A from HAP1 WT cell lysates. Rab1A in the starting material, unbound fraction, and precipitate was detected by WB using ab302545. IF was performed on WT and Rab1A KO cells. A signal was observed in both WT and KO cells using this antibody (1:100).

We highly encourage other scientists to share their observations.