Rab1A, Thermo Fisher Scientific, PA5-44578

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab1A and Rab1B and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Rab1A and Rab1B.

The collection of antibodies was tested using HAP1 WT, RAB1A KO and RAB1B KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution), PA5-44578 detects several intense unspecific bands (>24 kDa) in all cell lysates, including lysate from Rab1A KO cells. A weak band is observed at the expected size (22 kDa), however the band is of comparable intensity in all tested cell lysates. The antibody (2 µg) does not immunoprecipitate Rab1A from HAP1 WT cell lysates. Rab1A in the starting material, unbound fraction, and precipitate was detected by WB using ab302545. IF was performed on WT and Rab1A KO cells. A strong signal was observed in WT cells using this antibody (1:500), with some background signal in KO cells.

We highly encourage other scientists to share their observations.