Rab1A, Proteintech, 11671-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab1A and Rab1B and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Rab1A and Rab1B.

The collection of antibodies was tested using HAP1 WT, RAB1A KO and RAB1B KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 Ab dilution), 11671-1-AP detects a band of the expected size (22 kDa) in lysate from WT and Rab1B KO cells. The 22 band is markedly reduced but visible in Rab1A KO lysate. In addition, several intense unspecific bands can be observed above the 57 kDa marker in all cell lysates. The antibody (2 µg) does not immunoprecipitate Rab1A from HAP1 WT cell lysates. Rab1A in the starting material, unbound fraction, and precipitate was detected by WB using ab302545. IF was performed on WT and Rab1A KO cells. A strong signal was observed in both WT and KO cells using this antibody (1:700).

We highly encourage other scientists to share their observations.