Rab1A, Abcam, ab302545

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Rab1A and Rab1B and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Rab1A and Rab1B.

The collection of antibodies was tested using HAP1 WT, RAB1A KO and RAB1B KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 Ab dilution), ab302545 detects a band of the expected size (22 kDa) as well as a higher band of appr. 50 kDa in lysate from WT and Rab1B KO cells. The 22 kDa band is not observed in Rab1A KO lysate, and the 50 kDa band signal is markedly reduced in the Rab1A KO lysate. In addition, several unspecific bands are observed above the 57 kDa marker. The antibody (2 µg) enriches Rab1B signal from HAP1 WT cell lysates following immunoprecipitation. However, the band remains detectable in the unbound fraction, indicating that the target is not efficiently immunoprecipitated. Rab1B in the starting material, unbound fraction, and precipitate was detected by Western blot using the same antibody. IF was performed on WT and Rab1A KO cells. A clear signal was observed in WT cells using this antibody (1:100), with minimal background signal in KO cells.

We highly encourage other scientists to share their observations.