QPRTase, Thermo Fisher Scientific, MA5-25199

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Nicotinate-nucleotide pyrophosphorylase [carboxylating] and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 4 commercial research antibodies against human Nicotinate-nucleotide pyrophosphorylase [carboxylating] encoded by QPRT.

The collection of antibodies was tested using HAP1 WT and QPRT KO cells in the applications WB (WT and KO cell lysates and culture medium/30 µg protein), IP (WT cell lysate and culture medium) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) MA5-25199 detected no band in either cell culture og lysate. No band was detected following IP (2 ug ab used) in either cell lysate or culture medium. In IF (1:800 ab dilution), no staining was detected in either KO or WT cells.

We highly encourage other scientists to share their observations.