QPRTase, Thermo Fisher Scientific, MA5-25198

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Nicotinate-nucleotide pyrophosphorylase [carboxylating] and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 4 commercial research antibodies against human Nicotinate-nucleotide pyrophosphorylase [carboxylating] encoded by QPRT.

The collection of antibodies was tested using HAP1 WT and QPRT KO cells in the applications WB (WT and KO cell lysates and culture medium /30 µg protein), IP (WT cell lysate and culture medium) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) MA5-25198 detected a band of appr. 35 kDa in WT cell lysate and culture medium, with no corresponding band observed in KO cell lysate or culture medium. Lower molecular weight bands were observed in both genotypes in the culture medium and cell lysates, while higher molecular weight bands were detected in the culture medium for both genotypes. No band was detected following IP (2 ug ab used) in either cell lysate or culture medium. In IF (1:600 ab dilution), no staining was detected in either KO or WT cells.

We highly encourage other scientists to share their observations.