QPRTase, Abcam, ab171944

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Nicotinate-nucleotide pyrophosphorylase [carboxylating] and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 4 commercial research antibodies against human Nicotinate-nucleotide pyrophosphorylase [carboxylating] encoded by QPRT.

The collection of antibodies was tested using HAP1 WT and QPRT KO cells in the applications WB (WT and KO cell lysates and culture medium /30 µg protein), IP (WT cell lysate) and IF (WT and KO cells and culture medium) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) ab171944 detected a band of appr. 35 kDa in WT cell lysate and culture medium, with no corresponding band observed in KO cell lysate or culture medium. A band was also detected in both culture medium and cell lysate following IP (2 ug ab used). This band was markedly more intense than corresponding band in input extract (4% starting material) and no band was detected in immunodepleted extract (4% unbound fraction), showing that the antibody does deplete the antigen from both culture medium and cell lysate. In IF (1:200 ab dilution), a clear staining of WT cells was observed compared to KO cells showing no background.

We highly encourage other scientists to share their observations.