QPRTase, Proteintech, 25174-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Nicotinate-nucleotide pyrophosphorylase [carboxylating] and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 4 commercial research antibodies against human Nicotinate-nucleotide pyrophosphorylase [carboxylating] encoded by QPRT.

The collection of antibodies was tested using HAP1 WT and QPRT KO cells in the applications WB (WT and KO cell lysates and culture medium /30 µg protein), IP (WT cell lysate) and IF (WT and KO cells and culture medium) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) 25174-1-AP detected a band of appr. 35 kDa in both WT culture medium and lysate, with no corresponding band observed in KO cell lysate or culture medium. Bands of both higher and lower molecular weight was observed in culture medium and cell lysate of both genotypes. A band was also detected in both culture medium and cell lysate following IP (2 ug ab used). This band was markedly more intense than corresponding band in input extract (4% starting material) and no band was detected in immunodepleted extract (4% unbound fraction), showing that the antibody does deplete the antigen from both culture medium and cell lysate. In IF (1:800 ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.