Pleiotrophin, Aviva Systems Biology, ARP45356

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Pleiotrophin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 7 commercial research antibodies against human Pleiotrophin encoded by PTN.

The collection of antibodies was tested using U-87MG WT and PTN KO cells in the applications WB (WT and KO culture media/25 µg protein) and IP (WT culture media) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) ARP45356 detected no band. A band was detected following IP (2 µg antibody used); however, its intensity was only slightly increased compared to band in the input extract (2% of starting material) and the immunodepleted fraction (2% of the unbound material).

We highly encourage other scientists to share their observations.