Parkin, BioLegend, 865602

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human E3 ubiquitin-protein ligase parkin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human E3 ubiquitin-protein ligase parkin encoded by PRKN.

The collection of antibodies was tested using SH-SY5Y WT and PRKN KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (unknown dilution) 865602 detected a very faint band of appr. 50 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was also detected following IP (1 µg Ab used), this band was markedly more intense than corresponding band in input extract (10% of starting material), no band was detected in immunodepleted extract (10% of total unbound fraction), showing that the antibody does deplete the antigen from cell lysate. In IF (1:500 ab dilution), comparable staining intensity were observed in both WT and KO cells.

We highly encourage other scientists to share their observations.