Peroxiredoxin-6, Proteintech, 67499-1-lg

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Peroxiredoxin-6 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Peroxiredoxin-6 encoded by PRDX6.

The collection of antibodies was tested using HAP1 WT and PRDX6 KO cells in the applications WB (WT and KO cell lysates/25 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference). Furthermore, this antibody was tested in HeLa (WT and PRDX6 KO) cells in the application WB.

In WB (1:10000 ab dilution) using HAP1 cell lysate, 67499-1-lg detected a band of appr. 25 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. In Western blotting (1:20,000 antibody dilution), a band of approximately 25 kDa was detected in WT lysates from both HeLa and HAP1 cells, whereas no corresponding band was observed in KO lysates. A band was also detected following IP (2 µg Ab used). This band was markedly more intense than the corresponding band in input extract (10% of starting material). A very faint band was also detected in immunodepleted extract (10% of total unbound fraction). In IF (1:1000 ab dilution), a clear intense staining was observed in WT cells with no background staining in KO cells.

We highly encourage other scientists to share their observations.