Peroxiredoxin-1, Abcam, ab109506

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Peroxiredoxin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Peroxiredoxin-1encoded by PRDX1.

The collection of antibodies was tested using U2OS WT and PRDX1 KO cells in the applications WB (WT and KO cell lysates/10 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:5000 ab dilution) ab109506 detected a band of appr. 20 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was also detected following IP (1 μg Ab used); however, this band had same intensity as both input extract (10% of starting material) and immunodepleted extract (10% of total unbound fraction). In IF (1:250 ab dilution), a clear intense staining was observed in WT cells with no background signal in KO cells.

We highly encourage other scientists to share their observations.