PPP2R5D, Thermo Fisher Scientific, MA5-26647

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform by PPP2R5D.

The collection of antibodies was tested using HAP1 WT and PPP2R5D KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 ab dilution) MA5-26647 detected a band of appr. 65 kDa in WT cell lysate with a faint corresponding band observed in KO cell lysate. Furthermore, faint bands of both higher and lower molecular weight were observed in both genotypes. No band was detected following IP (2 μg Ab used). In IF (1:1000 ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.