PPP2R5D, Thermo Fisher Scientific, MA5-18066

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform by PPP2R5D.

The collection of antibodies was tested using HAP1 WT and PPP2R5D KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 ab dilution) MA5-18066 detected a band of appr. 65 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was also detected following IP (2 μg Ab used). This band was markedly more intense than the corresponding band in input extract (4% of starting material). Band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from the cell lysate. In IF (1:1000 ab dilution), stronger staining intensity was observed in KO cells compared to WT cells.

We highly encourage other scientists to share their observations.