Plectin, Abnova, H00005339-M03

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Plectin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human Plectin encoded by PLEC.

The collection of antibodies was tested using U2OS WT and KLEC KO cells in the applications WB (WT and KO cell lysates/10 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) H00005339-M03 detected multiple bands (>20) in both genotypes. Bands of appr. 520 and 530 kDa was specific to WT cell lysate, not observed in KO cell lysate. No band was detected following IP (1 µg Ab used). In IF (1:1000 Ab dilution), comparable staining intensities were observed in both KO and WT cell lysate.

We highly encourage other scientists to share their observations.