Serine/threonine protein kinase PINK 1, Bio-Techne, NBP2-36488

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Serine/threonine-protein kinase PINK1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Serine/threonine-protein kinase PINK1 encoded by PINK1.

The collection of antibodies was tested using HAP1 WT and PINK1 KO cells in the application WB (WT and KO cell lysates/30 µg protein) according to YCharOS standard protocols (see reference). Cells were treated with 10 µM of CCCP (Carbonyl cyanide 3- chlorophenylhydrazone) from MilliporeSigma (cat. number C2759) for 24h.

In WB (1:1000 ab dilution) NBP2-36488 detected a band at ~90 kDa and at ~40 kDa in both genotypes, which does not correspond to the expected molecular weight of 63 kDa.

We highly encourage other scientists to share their observations.