Profilin-1, Abcam, ab133529

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Profilin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human Profilin-1 encoded by PFN1.

The collection of antibodies was tested using HAP1 WT and PFN1 KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:10000 ab dilution) ab133529 detected a band of appr. 15 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. No band was detected following IP (2 μg ab used). In IF (1:60 ab dilution), comparable staining intensity was observed in KO and WT cells.

We highly encourage other scientists to share their observations.