Profilin-1, Thermo Fisher Scientific, MA5-25120

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Profilin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human Profilin-1 encoded by PFN1.

The collection of antibodies was tested using HAP1 WT and PFN1 KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:2000 ab dilution) MA5-25120 detected a band of appr. 15 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. No band was detected following IP (2 μg ab used). In IF (1:600 ab dilution), a clear intense staining was observed in WT cells with no background staining in KO cells.

We highly encourage other scientists to share their observations.