Optineurin, Structural Genomics Consortium, Z-OPTN-10

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Optineurin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human Optineurin encoded by OPTN.

The collection of antibodies was tested using U2OS WT and OPTN KO cells in the applications WB (WT and KO cell lysates/35 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) Z-OPTN-10 detected no band. A band was detected following IP (2 µg Ab used). This band had same intensity as corresponding band in input extract (4% of starting material). Band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from the cell lysate. In IF (1:500 ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.