Optineurin, Bio-Techne, NBP3-19900

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Optineurin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human Optineurin encoded by OPTN.

The collection of antibodies was tested using U2OS WT and OPTN KO cells in the applications WB (WT and KO cell lysates/35 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) NBP3-19900 detected a strong band of appr. 66 kDa in WT cell lysate, with no corresponding band observed in KO cell lysate. Several faint bands of higher and lower molecular weight were observed in KO cell lysate. A band was detected following IP (2 µg Ab used). This band was markedly more intense than the corresponding band in input extract (4% of starting material). No band was detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does completely deplete the antigen from the cell lysate. In IF (1:300 ab dilution), a clear intense staining was observed in WT cells with little to no background staining in KO cells.

We highly encourage other scientists to share their observations.