Netrin-1, Thermo Fisher Scientific, PA5-98017

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Netrin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Netrin-1 encoded by NTN1.

The collection of antibodies was tested using MCF7 WT and NTN1 KO cells in the applications WB (WT and KO cell culture medium/10 µg protein) and IP (WT culture medium) according to YCharOS standard protocols (see reference).

In Western blot (1:3000 antibody dilution), PA5-98017 detected a band at approximately 75 kDa in both genotypes, with higher intensity in KO culture medium. A lower molecular weight band was also observed in both genotypes. No band was detected following IP (2 ug Ab used).

We highly encourage other scientists to share their observations.